![]() Kim DH (2013) Bacteria and the aging and longevity of Caenorhabditis elegans. J Gerontol A Biol Sci Med Sci 55:215–219ĭas R, Melo JA, Thondamal M et al (2017) The homeodomain-interacting protein kinase HPK-1 preserves protein homeostasis and longevity through master regulatory control of the HSF-1 chaperone network and TORC1- restricted autophagy in Caenorhabditis elegans. Gems D, Riddle DL (2000) Defining wild-type life span in Caenorhabditis elegans. Ĭornwell AB, Llop JR, Salzman P et al (2018) The replica set method: a high-throughput approach to quantitatively measure Caenorhabditis elegans lifespan. Cold Spring Harb Symp Quant Biol 72:489–497. Samuelson AV, Klimczak RR, Thompson DB et al (2007) Identification of Caenorhabditis elegans genes regulating longevity using enhanced RNAi-sensitive strains. Samuelson AV, Carr CE, Ruvkun G (2007) Gene activities that mediate increased life span of C. Mantel N (1966) Evaluation of survival data and two new rank order statistics arising in its consideration. Kaplan EL, Meier P (1958) Nonparametric estimation from incomplete observations. Raj F, Amrit G, Ratnappan R et al (2014) The C. Klass MR (1977) Aging in the nematode Caenorhabditis elegans: major biological and environmental factors influencing life span. Timmons L, Fire A (1998) Specific interference by ingested dsRNA. Johnson T (1990) Increased life-span of age-1 mutants in Caenorhabditis elegans and lower Gompertz rate of aging. elegans mutant that lives twice as long as wild type. Kenyon C, Chang J, Gensch E et al (1993) A C. Statistical analysis of quantitative phenotypes.The protocols provided here describe both the traditional experimental approach and the Replica Set method, as well as practical considerations for each. Unlike traditional approaches, the Replica Set method centers on assaying independent samples of a population at each observation point, rather than a single sample over time as with “traditional” longitudinal methods. The method requires common equipment found in most laboratories working with C. The replica set method allows a single investigator to screen more treatments or conditions in the same amount of time without loss of data quality. We previously developed an alternative approach, called the Replica Set method, to quantitatively measure lifespan of C. A common approach to measure Caenorhabditis elegans lifespan is to follow a population of animals over time and score viability based on movement. Lifespan is the most straightforward surrogate measure of aging, as it is easily quantifiable.
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